Fabian Coscia (Presenting Author) | Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC) | Robert-Rössle-Straße 10, 13125 , Berlin, Germany
Abstract
Spatial proteomics (SP) is transforming our ability to understand the location, abundance, and interactions of proteins within cells and tissues (Method of the Year 2024, Nature Methods), offering profound insights into how proteins influence both normal and disease processes. Multiscale strategies that integrate targeted (imaging-based) and exploratory (LC-MS-based) methods are becoming increasingly popular for biomarker and drug target discovery. Our team has co-developed deep visual proteomics (DVP), which combines high-resolution microscopy, AI-driven image analysis, and laser microdissection-guided ultrasensitive proteomics. In my presentation, I will present an overview of our spatial tissue proteomics and DVP pipelines, discuss current efforts to advance throughput and sensitivity, and outline their translational potential in cancer and neurobiology, down to the single-cell level.
