Home » Abstracts » EuPA initiatives - Full Day Session » Proteomics Multicentric Experiment 13 (PME13): EuPA standardization initiative: High sensitivity proteomics.

Proteomics Multicentric Experiment 13 (PME13): EuPA standardization initiative: High sensitivity proteomics.

EuPA initiatives - Full Day Session

Abstract #273 Posted on28 March 202512 May 2025 12 May 2025 12h21

Author(s)

Marina Gay (Presenting Author) | Institute for Research in Biomedicine of Barcelona (IRB Barcelona), Barcelona Institute of Science and Technology (BIST) | C/ Baldiri Reixac 10, 08028, Barcelona, Spain
Gianluca Arauz | Institute for Research in Biomedicine of Barcelona (IRB Barcelona), Barcelona Institute of Science and Technology (BIST) | C/ Baldiri Reixac 10, 08028, Barcelona, Spain
Ignasi Folch-I-Casanovas | Institute for Research in Biomedicine of Barcelona (IRB Barcelona), Barcelona Institute of Science and Technology (BIST) | C/ Baldiri Reixac 10, 08028, Barcelona, Spain
Mikel Azkargota | CIC bioGUNE, Basque Research and Technology Alliance (BRTA), CIBERehd | Science & Technology Park of Bizkaia, buid. 800, 48160, Derio, Spain
Anne Gonzalez de Peredo | Proteomics Facility of Toulouse, CNRS | 205, route de Narbonne, 31077, Toulouse, France
Karima Chaoui | Proteomics Facility of Toulouse, CNRS | 205 route de Narbonne, BP 64182, 31077, Toulouse, France
Valerianne Gaxotte | University of Strasbourg | 25 Rue Becquerel, 67200, Strasbourg, France
Christine Carapito | University of Strasbourg | 25 Rue Becquerel, 67200, Strasbourg, France
Ferdinando Cerciello | University Hospital of Bern | Freiburgstrasse 18, 3010, Bern, Switzerland
Sergio Ciordia | CNB Proteomics Facility; Centro Nacional de Biotecnología (CNB-CSIC) | C/Darwin 3. Campus de UAM, 28049 , Madrid, Spain
Odile Burlet-Schiltz | Proteomics Facility of Toulouse, CNRS | 205 route de Narbonne, BP 64182, 31077, Toulouse, France
Felix Elortza | CIC bioGUNE, Basque Research and Technology Alliance (BRTA), CIBERehd | Science & Technology Park of Bizkaia, buid. 800, 48160, Derio, Spain
Fernando J. Corrales | CNB Proteomics Facility; Centro Nacional de Biotecnología (CNB-CSIC) | C/Darwin 3. Campus de UAM, 28049 , Madrid, Spain

Abstract

Single cell analysis at the proteome level has become feasible as new technologies for sample preparation and mass spectrometry analysis ensure efficient protein extraction and processing, as well as analytical sensitivity in the attomolar range. However, the implementation of high sensitivity proteomics (HSP) protocols remains a challenge. In this context, the EuPA standardisation initiative launched the PME13 with the main objectives of benchmarking HSP analysis protocols, providing tools to facilitate HSP implementation and assessing laboratory performance. The sample used in the study consisted of a serial dilution of E. coli protein extract from 100 to 1 ng, provided to participants in intact and trypsin digested formats. A pilot analysis was conducted to optimise the analytical pipeline and to minimize sources of uncertainty. After analysis, datasets were submitted for centralised processing along with metadata specific to each participant. Thirty-six laboratories from eight different countries requested PME13 samples, almost half of which (17 out of 36) had not participated in previous PMEs. Overall, our data indicate that the reference sample provides a reliable basis for benchmarking, establishing protocols and assessing performance. The influence of analytical variables and the correlation with the physico-chemical properties of the analysed peptides are presented and discussed.

Abstract N°

Review committee

Sélectionnez le(s) utilisateur(s) chargé(s) de la revue de cet abstract.

Communication type

Speaker abstract
Oral presentation
Flash communication
Poster

Communication status

Accepted
Rejected

Final abstract N°

Note globale

Notification mail sent

Abstract content *

Max 2000 characters. Line breaks are supported.

Author(s) informations *

1	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
2	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
3	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
4	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
5	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
6	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
7	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
8	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
9	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
10	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
11	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
12	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
13	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
1	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *

Add another author

Are you an ECR? *

<7 years after last degree

Are you a Junior PI? *

If you have started your own group less than 3 years ago and are not chosen by the scientific committee for a talk, you will have the opportunity to give a keynote during an ECR parallel session

Are you an invited speaker? *

Consent *

By submitting this form I agree
- that the administrators use my personal information for the abstract revision process.
- that my name, affiliation and abstract content may be publicly displayed.

Captcha