Home » Abstracts » Data processing for Quantitative Proteomics » Histone Derivatization with Trimethylacetic Anhydride for Enhanced Characterization of Glucose-Sensitive Acetylation Sites via Isotope Tracing

Histone Derivatization with Trimethylacetic Anhydride for Enhanced Characterization of Glucose-Sensitive Acetylation Sites via Isotope Tracing

Data processing for Quantitative Proteomics PTM & Glycoproteomics

Abstract #101 Posted on18 March 20259 May 2025 9 May 2025 14h19

Abstract

Histone post-translational modifications (hPTMs) play a crucial role in gene expression regulation and are closely linked to cellular metabolism. Histones are rich in lysine (K) and arginine (R) residues, and are generally chemically derivatized before tryptic digestion to modify all unmodified and monomethylated lysine residues. Then Arg-C-like peptides are produced, that are longer, less hydrophilic, and of same sequence whatever the PTM status of lysines.
Here, we optimized a derivatization protocol using trimethylacetic anhydride (TMA), a recently described reagent that improves the separation of positional isomers compared to the commonly used propionic anhydride. Our streamlined protocol increases labeling efficiency while reducing the undesired derivatization of serine and threonine residues. Additionally, we found that TMAylation influences the estimated relative abundance of variably modified peptides, particularly favoring acetylated peptides.
We applied our TMAylation protocol to investigate histone lysine acetylation dynamics in the mouse brain following in vivo injection of 13C-labeled glucose. To facilitate the analysis, we developed a bioinformatics workflow to process the resulting MS data which exhibit enlarged isotopic distributions, particularly for multiply acetylated peptides. Using TMA enables precise measurement of heavy acetyl incorporation with unprecedented site-specific resolution, allowing for the detailed characterization of dynamically acetylat

Abstract N°

Review committee

Sélectionnez le(s) utilisateur(s) chargé(s) de la revue de cet abstract.

Communication type

Speaker abstract
Oral presentation
Flash communication
Poster

Communication status

Accepted
Rejected

Final abstract N°

Note globale

Abstract content *

Max 2000 characters. Line breaks are supported.

Histone post-translational modifications (hPTMs) play a crucial role in gene expression regulation and are closely linked to cellular metabolism. Histones are rich in lysine (K) and arginine (R) residues, and are generally chemically derivatized before tryptic digestion to modify all unmodified and monomethylated lysine residues. Then Arg-C-like peptides are produced, that are longer, less hydrophilic, and of same sequence whatever the PTM status of lysines. 
Here, we optimized a derivatization protocol using trimethylacetic anhydride (TMA), a recently described reagent that improves the separation of positional isomers compared to the commonly used propionic anhydride. Our streamlined protocol increases labeling efficiency while reducing the undesired derivatization of serine and threonine residues. Additionally, we found that TMAylation influences the estimated relative abundance of variably modified peptides, particularly favoring acetylated peptides. 
We applied our TMAylation protocol to investigate histone lysine acetylation dynamics in the mouse brain following in vivo injection of 13C-labeled glucose. To facilitate the analysis, we developed a bioinformatics workflow to process the resulting MS data which exhibit enlarged isotopic distributions, particularly for multiply acetylated peptides. Using TMA enables precise measurement of heavy acetyl incorporation with unprecedented site-specific resolution, allowing for the detailed characterization of dynamically acetylat

Author(s) informations *

1	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
2	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
3	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
4	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
5	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
6	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *
1	First name * Last name * email * Presenting author * At least and only one presenting author must be selected per abstract Presenting author Do you want this email to appear in the book of abstracts? If you refuse, only the administrators and the review commitee will have access to this email as part of the review process. YesNo Laboratory * Address * ZIP code * City * Country *

Add another author

Are you an ECR? *

<7 years after last degree

Are you a Junior PI? *

If you have started your own group less than 3 years ago and are not chosen by the scientific committee for a talk, you will have the opportunity to give a keynote during an ECR parallel session

Are you an invited speaker? *

Consent *

By submitting this form I agree
- that the administrators use my personal information for the abstract revision process.
- that my name, affiliation and abstract content may be publicly displayed.

Captcha